Influenza Pseudotyped Reporter Virus Particles
✔Stable HA sequence
Integral Molecular’s Influenza Reporter Virus Particles (RVPs) are replication-incompetent pseudotyped virus particles that enable safe (BSL-2), easy, and high-throughput viral infectivity and neutralization assays using standard detection instrumentation. The RVPs display hemagglutinin (HA) and neuraminidase (NA) proteins on a heterologous virus core and carry a modified genome that expresses a convenient optical reporter gene (GFP or luciferase).
Conventional hemagglutination and microneutralization assays require handling of live and sometimes highly pathogenic BSL-3 viruses that are subject to drift upon propagation. In contrast, Influenza RVPs are a phenotypically stable, safe, and ready-to-use reagent.
RVP Readouts: agglutination and infection
A) Influenza RVPs containing HA and NA proteins agglutinated red blood cells upon incubation for 1 hour. B) Infection of HEK-293T cells with Influenza RVPs was inhibited by a neutralizing monoclonal antibody (Sino #68031-H011). A non-neutralizing control antibody did not inhibit infection.