Influenza Pseudotyped Reporter Virus Particles

Influenza Components

 

Applications

Antibody neutralization
Serum screening
High-throughput assays

Advantages

BSL-2 use 
Stable HA sequence 
Quantitative readout


Influenza RVP catalog

Influenza A RVPs


Catalog No. Virus (Subtype)  Strain Information
RVP-1201 Influenza A (H5N1) Indonesia/5/05

 

Influenza B RVPs


Catalog No. Virus (Lineage)  Strain Information
RVP-1301 Influenza B (Victoria) Washington/02/2019

 

SEE FULL RVP CATALOG

Integral Molecular’s Influenza Reporter Virus Particles (RVPs) are replication-incompetent pseudotyped virus particles that enable safe (BSL-2), easy, and high-throughput viral infectivity and neutralization assays using standard detection instrumentation. The RVPs display hemagglutinin (HA) and neuraminidase (NA) proteins on a heterologous virus core and carry a modified genome that expresses a convenient optical reporter gene (GFP or luciferase).

Conventional hemagglutination and microneutralization assays require handling of live and sometimes highly pathogenic BSL-3 viruses that are subject to drift upon propagation. In contrast, Influenza RVPs are a phenotypically stable, safe, and ready-to-use reagent.


RVP Readouts: agglutination and infection

Agglutination and Neut, Influenza

 

A) Influenza RVPs containing HA and NA proteins agglutinated red blood cells upon incubation for 1 hour. B) Infection of HEK-293T cells with Influenza RVPs was inhibited by a neutralizing monoclonal antibody (Sino #68031-H011). A non-neutralizing control antibody did not inhibit infection.

LEARN MORE ABOUT RVPS