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The Problem

  • Soluble envelope glycoprotein (Env) trimers are attractive HIV-1 vaccine candidates, but their utility is limited by low stability
  • We used comprehensive mutagenesis to engineer Env trimers to generate variants with increased stability

The Solution

High-Throughput Protein Engineering Improves the Antigenicity and Stability of Soluble HIV-1 Envelope Glycoprotein SOSIP Trimers

We created and screened 852 variants to engineer a protein that retained structural integrity and bound neutralizing conformational antibodies
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The Impact HIV case study_structure

  • We identified 29 Env trimer variants with antigenic improvements
  • The improved trimer variants identified here may be useful to further vaccine discovery
Harness Integral Molecular’s 20+ years of protein engineering experience to improve gene therapy using GeneCanvas
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